Figure 6.

Specific blockade of TrkA by siRNA decreases cell viability and proliferation and downregulates the phosphorylation of survival‐promoting proteins in NPM‐ALK ⁺ T‐cell lymphoma cells. Transfection of TrkA siRNA decreased the (A) viability and (B) proliferation of SR‐786 cells. Data represent means ± SE of three independent experiments (*P < 0.001 vs. scrambled siRNA‐transfected cells). (C) The decrease in TrkA was associated with decreased phosphorylation of NPM‐ALK, IGF‐IR, AKT, and STAT3. β‐Actin was used as the loading control. MW: TrkA and pTrkA, 140 kDa; NPM‐ALK and pNPM‐ALK, 80 kDa; IGF‐IR and pIGF‐IR, 95 kDa; AKT and pAKT, 60 kDa; STAT3 and pSTAT3, 86 kDa.