Table 3. Relative hsp16-GFP fusion protein levels in various genetic backgrounds as determined by GFP fluorescence.

Strains	25°C	36°C (4 h)
hsp16–GFP	12.5 ± 4.4	48 ± 6.6
cdc22-M45 hsp16–GFP	22.2 ± 3.3	122 ± 1.5
rad1-1	19.4 ± 1.4	51 ± 1.5
Δspc1 hsp16–GFP	16.5 ± 5.2	42.0 ± 1.1
Δatf1 hsp16–GFP	5.3 ± 1.4	19.5 ± 1.3
Δwis1 hsp16–GFP	19.8 ± 1.9	71.2 ± 2.2
Δpcr1 hsp16–GFP	11.3 ± 1.9	34.5 ± 1.3
Δras1 hsp16–GFP	8.8 ± 0.4	53.8 ± 6.6
rad1-1 cdc22-M45 hsp16–GFP	15.0 ± 1.3	30 ± 2.5
Δspc1 cdc22-M45 hsp16–GFP	16.0 ± 5.5	43.5 ± 2.4
Δatf1 cdc22-M45 hsp16–GFP	9.5 ± 3.2	53.2 ± 3.0
Δwis1 cdc22-M45 hsp16–GFP	42.1 ± 2.3	124.2 ± 1.4
Δpcr1 cdc22-M45 hsp16–GFP	19.1 ± 3.8	121 ± 0.6