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. 2017 Aug 9;18(9):1646–1659. doi: 10.15252/embr.201643581

Figure 6. Enforced expression of circHIPK3 suppresses the growth, metastasis, and angiogenesis of bladder cancer cells in vivo .

Figure 6

  • A, B
    Hypodermic injection of T24T cells stably transfected with circHIPK3 or vector control into BALB/c nude mice established subcutaneous xenograft tumors (5 × 106 cells per mouse, n = 6 for each group). Compared with vector group, the tumor growth rate and weight significantly decreased in circHIPK3‐treated nude mice. Data are mean ± SEM, **P < 0.01 (Student's t‐test).
  • C
    Immunohistochemical staining revealed that over‐expression of circHIPK3 led to decreased expression of HPSE, MMP‐9, VEGF, and CD31 within tumors. Stable transfection of circHIPK3 reduced the mean vessel density within tumors. Data are mean ± SEM, **P < 0.01 (Student's t‐test). Scale bars, 50 and 100 μm.
  • D
    T24T cells stably transfected with circHIPK3 or vector control were injected into the tail vein of BALB/c nude mice (2 × 106 cells per mouse, n = 6 for each group). The nude mice treated with circHIPK3 transfected cells demonstrated significantly fewer lung metastatic colonies. Data are mean ± SEM (Student's t‐test). Scale bars, 100 and 200 μm.