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. 2017 Aug 4;49(8):e362. doi: 10.1038/emm.2017.107

Figure 4.

Figure 4

dNP2-ctCTLA-4 reduces Th2 responses, such as eosinophil infiltration and IL-5 production. Bronchoalveolar lavage fluids were collected, and the cells were stained with a Hemacolor staining kit after cytocentrifugation on slide glasses. The slides were observed on bright-field microscopy. (a) Total cell numbers were counted. (b) The number of macrophages were counted. (c) The numbers of eosinophils were counted. (d) The supernatants of bronchoalveolar lavage after centrifugation were collected, and the production of IL-5, IL-13 and IFN-γ was analyzed using an ELISA kit. (e) The lungs were harvested, and tissues were homogenized on ice. The concentration of IL-5, IL-13, IL-4 and IFN-γ was analyzed using an ELISA kit, and the values were normalized to the total protein concentration of the tissues, which was quantitated by Bradford assay. n=5 per group and results are presented as the mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001.