Table 2. Preparative Scale Production of AO Metabolites Using ecoAOa.
substrate | mM –1 cm –1 | metabolite | mM–1 cm –1 | % yield |
---|---|---|---|---|
zoniporide | ε315 6.48 ± 0.04 | 2-oxo-zoniporide | ε280 5.48 ± 0.15 | 10 |
DACA | ε358 10.48 ± 0.09 | DACA-acridone | ε409 9.24 ± 0.07 | 25 |
zaleplon | ε333 7.04 ± 0.03 | 5-oxo-zaleplon | ε330 7.70 ± 0.26 | 10 |
O6-BG | ε282 9.10 ± 0.05 | 8-oxo-O6-BG | ε290 9.50 ± 0.22 | 12 |
All extinction coefficients were determined in buffer (pH 7.4) using an Agilent 8453 UV–vis spectrophotometer (Agilent Technologies, Santa Clara, CA). The error is reported as the SD for duplicate measurements.