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. 2017 Aug 3;65(34):7542–7552. doi: 10.1021/acs.jafc.7b02557

Figure 2.

Figure 2

Identification of transamidated sites induced by mTG using LTQ-FT Ultra. (A) Observed fragment of DQ2.5-glia-α1a before treatment (upper spectrum) and after treatment with mTG in the presence of Lys (lower spectrum); predicted fragment ion masses are given in the table with identified ions illustrating transamidation shown in boxes. (B) Observed fragment of DQ2.5-glia-α2 before treatment (upper spectrum) and after treatment with mTG in the presence of Lys (lower spectrum); predicted fragment ion masses are given in the table with identified ions illustrating transamidation shown in boxes. Arrows indicate the shift in Da due to transamidation.