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. 2017 Aug 3;65(34):7542–7552. doi: 10.1021/acs.jafc.7b02557

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Copyright © 2017 American Chemical Society

This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

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Reactivity pattern of glia-α1and glia-α2-specific-T-cell clones to DQ2.5-glia-α1a, DQ2.5-glia-α2, 33-mer, gliadin, and their mTG-treated forms. (A) Reactivity pattern of glia-α1-specific T-cell clone S2 and L5107 to DQ2.5-glia-α1a and its modified products (5 concentrations tested in each group). (B) Reactivity pattern of glia-α2-specific T-cell clones S16 and D1 to DQ2.5-glia-α2 and its modified products (5 concentrations tested with S16 and only the highest concentration with D1). (C) Reactivity pattern of glia-α1-specific T-cell clones S2, and L5107 to 33-mer and its modified products (5 concentrations tested in each group). (D) Reactivity pattern of glia-α2-specific T-cell clones S16 and D1 to 33-mer and its modified products (5 concentrations tested with S16 and only the highest concentration with D1). (E) Reactivity pattern of glia-α1-specific T-cell clones L6 and L10 to DQ2.5-glia-α1a, 33-mer and their modified products (only the highest concentration tested). (F) Reactivity pattern of glia-α2-specific T-cell clones 101136 and L3-13 to DQ2.5-glia-α2, 33-mer and their modified products (only the highest concentration tested). (G) Reactivity pattern of glia-α1-specific T-cell clones to gliadin protein and its modified products (only the highest concentration tested). (H) Reactivity pattern of glia-α2-specific T-cell clones to gliadin protein and its modified products (only the highest concentration tested). PC, deamidated peptides as positive control; NC, native peptides as negative control. Relative response: CPM of T cell clone caused by peptide/that of caused by deamidated peptide (positive control).