Figure 3.

(A) Stimulation of cells with 40–300 µM cyanidin increased intracellular Ca²⁺ in a concentration-dependent manner; (B) 100 µM Cyanidin resulted in a 33–35% increase in intracellular Ca²⁺ compared to 2 µM ionomycin; (C) Removal of extracellular Ca²⁺ abolished the effect of cyanidin, but not after endoplasmic reticulum (ER) store depletion with thapsigargin. Under extracellular Ca²⁺ free and store depletion conditions, the cyanidin response is also abolished. Results are presented as average traces or mean + S.E.M.; n = 89–162 cells/group from the three independent experiments; * p < 0.05 when compared with the control.