. 2017 Aug 17;9(8):893. doi: 10.3390/nu9080893

Table 2.

Effects of omega–3 oil supplementation and breed on heart fatty acid profile of prime lambs.

Item ¹		Treatment				Breed ²			SEM ³	p Value ⁴
Item ¹	Control	2.5C	5C	2.5F	5F	M × M	C × M	W × C		T	B	T × B
Total lipid (g fat/100 g wet tissue)	2.6	2.7	2.8	2.9	2.4	2.8	2.7	2.5	0.09	0.35	0.23	0.16
Percentage (g/100 g total FA)
14:0	0.6	0.6	0.8	0.6	0.5	0.6	0.7	0.5	0.05	0.68	0.33	0.24
15:0	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.01	0.36	0.94	0.30
16:0	12.9	12.5	13.1	12.9	12.7	12.9	12.9	12.7	0.22	0.93	0.63	0.06
17:0	1.1 ^a	1.0 ^a,b	1.0 ^a,b	0.9 ^a,b	0.8 ^b	1.1 ^a	1.0 ^a,b	0.9 ^b	0.03	0.04	0.02	0.07
18:2n-6	23.8	21.0	21.0	21.9	22.4	20.9	23.1	22.9	0.48	0.11	0.06	0.07
18:3n-3	2.0	1.8	2.5	2.1	2.7	2.5	2.1	1.7	0.18	0.53	0.24	0.56
18:1n-9	13.4	15.5	15.6	14.2	12.8	14.9	14.7	12.9	0.45	0.17	0.08	0.39
18:0	17.2	18.4	18.4	17.9	17.5	18.3	17.3	17.6	0.27	0.35	0.12	0.06
20:4n-6 (ARA)	5.6	6.2	4.3	5.8	5.2	5.1	4.8	6.5	0.33	0.33	0.06	0.06
20:5n-3 (EPA)	1.2	1.3	1.2	1.3	1.4	1.2	1.3	1.3	0.04	0.35	0.33	0.07
20:3n-6	0.5	0.4	0.4	0.4	0.5	0.4	0.4	0.5	0.01	0.22	0.37	0.44
20:4n-3	1.4	1.4	1.5	1.6	1.7	1.4	1.5	1.6	0.06	0.65	0.47	0.49
20:2n-6	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.01	0.81	0.77	0.91
20:0	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.01	0.99	0.74	0.87
22:5n-6	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.01	0.25	0.23	0.23
22:6n-3 (DHA)	0.6	0.7	0.7	0.7	0.7	0.7	0.6	0.7	0.03	0.85	0.56	0.72
22:4n-6	0.2	0.2	0.1	0.2	0.2	0.1	0.1	0.2	0.01	0.72	0.20	0.57
22:5n-3 (DPA)	1.1	1.1	1.2	1.3	1.4	1.2 ^b	1.3 ^a,b	1.4 ^a	0.05	0.19	0.04	0.02
22:0	0.3	0.3	0.2	0.3	0.3	0.3	0.3	0.3	0.01	0.74	0.22	0.83
23:0	0.3	0.3	0.3	0.3	0.3	0.3	0.3	0.3	0.01	0.37	0.56	0.74
24:0	0.3	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.01	0.85	0.46	0.67
∑SFA	34.5	35.1	35.9	35.2	34.2	35.5	34.5	34.8	0.46	0.63	0.31	0.01
∑MUFA	24.3	26.3	27.3	25.3	24.8	26.7 ^a	25.5 ^a,b	23.8 ^b	0.50	0.27	0.04	0.30
∑PUFA	41.2	38.6	36.8	39.5	41.0	37.8	40.0	41.4	0.87	0.30	0.08	0.03
PUFA/SFA	1.2	1.2	1.0	1.2	1.2	1.1	1.2	1.2	0.04	0.52	0.06	0.01
∑n-3 PUFA	6.5	6.4	6.9	6.6	7.9	7.0	6.7	6.7	0.25	0.36	0.90	0.26
∑n-6 PUFA	30.3	28.1	26.1	28.6	28.5	26.9^b	28.7 ^a,b	30.3 ^a	0.68	0.17	0.02	0.06
n-6/n-3	4.8 ^a	4.4 ^a,b	4.1 ^a,b	4.3 ^a,b	3.6 ^b	4.0	4.4	4.6	0.15	0.02	0.28	0.83
Content (mg/100 g wet tissue)
18:3n-3	24.1	26.7	24.6	27.5	31.0	31.7	18.7	26.7	2.19	0.83	0.09	0.81
20:4n-6	77.1	101.7	59.9	68.8	71.0	68.6	60.4	99.0	7.44	0.55	0.08	0.58
EPA	13.7	15.7	15.4	18	20.5	16.1	13.1	20.3	1.37	0.64	0.07	0.56
22:5n-6	1.1	1.1	1.2	1.1	0.9	1.0	1.0	1.3	0.09	0.89	0.23	0.94
DHA	7.8	8.0	8.1	9.3	10.4	9.2	6.4	10.1	0.71	0.79	0.14	0.90
DPA	14.0	15.3	16.1	18.0	20.0	15.3	13.4	20.6	1.37	0.72	0.07	0.58
EPA + DHA	21.5	23.7	23.5	27.3	30.9	25.3	19.5	30.4	2.02	0.67	0.09	0.72
EPA + DHA + DPA	35.5	39.0	39.6	45.3	50.9	40.6	32.9	51.0	3.36	0.69	0.08	0.66

¹ All definitions and abbreviations are as indicated in Table 1; FA: fatty acid; ARA: arachidonic acid; EPA: eicosapentaenoic acid; DPA: docosapentaenoic acid and DHA: docosahexaenoic acid; ² M × M: purebred Merino; C × M: Corriedale and Merino crossbred; W × C: White Suffolk and Corriedale crossbred; ³ SEM: standard error of the mean; ⁴ T: treatment; B: breed; T × B: treatment × breed. Row means bearing different superscripts within a fixed factor differ significantly (p < 0.05). In all lamb tissues (Table 2, Table 3 and Table 4), low levels of trans 18:1 isomers and conjugated linoleic acid (CLA) isomers were also present.