Figure 1.

Design of the study. Female C57BL/6J mice were divided into four groups and fed the normal fat (NF) normal choline (CO) diet, NF choline supplemented (CS) diet, HFCO diet, or HFCS diet for 6 weeks before timed-mating and throughout gestation. Male mice followed the NFCO diet until timed-mating. The intraperitoneal glucose tolerance test (GTT) was conducted at embryonic day (E) 15.5 and dissection was conducted at E17.5. n is the number of dams in each group from which data and samples were collected.