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Western blot analysis. L4717, YBH3 and strains bearing nop2 ts alleles were shifted from 25 to 37°C and grown at 37°C for 6 h. Total cell protein extracts were prepared and analyzed by western blotting with an affinity-purified antipeptide polyclonal antibody directed against Nop2p (APpAb3). After stripping immunoglobulins from the membrane, it was reprobed with a monoclonal antibody (mAb D68). Nop2p (arrowhead) and non-specific protein bands (asterisks) are indicated.
