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. 2001 Jul 15;29(14):2927–2937. doi: 10.1093/nar/29.14.2927

Figure 4.

Figure 4

mAb D68 recognizes Nop2p. (a–d) Immunofluorescence localization in strain L4717. mAb D68 co-localizes with anti-Nop2p affinity-purified antipeptide polyclonal antibody 3 (APpAb3; 8). Counterstaining of chromatin with DAPI and a phase contrast image of the same field are shown. (e–h) Detection of Nop2p with mAb D68 in strain YBH5 (GAL::NOP2; 14). YBH5 was shifted from YPG to YPD, grown for 1, 3, 6 or 12 h and analyzed by immunofluorescence localization with mAb D68. Panels are overexposed (by 2-fold compared with a) to reveal a low level of non-specific background staining in yeast cell cytoplasm. Bar, 5 µm.