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. 2001 Jul 15;29(14):2927–2937. doi: 10.1093/nar/29.14.2927

Figure 5.

Figure 5

Polysome analysis. Strains bearing nop2 ts alleles and YBH3 were grown at 25°C, shifted to 37°C or maintained at 25°C and grown for 2 h. YBH23 (nop2-10) was also shifted to medium containing 3% formamide (+ FA) and grown for 2 h at 25°C. Cell extracts were layered onto 15–50% sucrose gradients, centrifuged and analyzed using absorbance at 254 nm. See panel O for positions of ribosome subunits (40S and 60S), monosomes (80S) and polysomes (2–6). H, halfmer 43S preinitiation complexes are labeled.