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Mapping mutations in nop2-4. Restriction sites BsgI and MfeI were used to subclone regions of nop2-4 into a NOP2 plasmid to create alleles nop2-41 through nop2-46, which were introduced into a nop2::LEU2 strain (YBH3) by plasmid shuffling (Table 1). Serial dilutions of the resulting strains were replica plated onto YPD and grown at 25 or 37°C for 3 days. Asterisks represent approximate positions of amino acid substitutions in mutant alleles.
