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. 2017 Aug 22;9(8):915. doi: 10.3390/nu9080915

Figure 4.

Figure 4

Effect of NP and RP on the expression of iNOS, COX-2 and nitrite levels. Western blot analysis for iNOS and COX-2 demonstrated a significant increased levels after LPS stimulation (a,b); treatments with NP only at concentrations of 0.1 and 0.5 mg/mL and RP at 0.01 and 0.1 mg/mL significantly reduced iNOS expressions. No protection was observed with NP 0.01 mg/mL. The levels of COX-2 were also reduced with NP treatment at concentrations of 0.1 and 0.5 mg/mL and RP at 0.01 and 0.1 mg/mL. Less protection, but significant, was observed with NP 0.01 mg/mL. Moreover, we analyzed the levels of nitrite production and we observed an increase of nitrite levels after LPS stimulation (c), whereas treatments with NP, only at 0.5 mg/mL, significantly reduced nitrite production. Moreover, we determinate the levels of ROS content and MDA production (d,e), and observed an increase of ROS content and MDA levels after LPS stimulation, whereas treatments with NP and RP reduced ROS and MDA levels at concentration dependent manner. Data are representative of at least three independent experiments; *** p < 0.001 vs. Ctr; ### p < 0.001 vs. LPS; ## p < 0.01 vs. LPS.