Figure 2.

Polyphenols recover H₂O₂-induced HUVEC (human umbilical cord vein endothelial cells) proliferation and apoptosis. (A) HUVEC proliferation in response to H₂O₂ with/without HT + Tyr (48 h). Data are reported as cells/well ± SEM (n = 3 run in triplicate). Ctr = 1% FCS; ^a p < 0.05, ^b p < 0.01, ^c p < 0.001 versus Ctr, ^d p < 0.01 versus H₂O₂ 100 µM; (B) Cleaved caspase-3 in HUVEC exposed to H₂O₂ (100 µM) for 6 h, with/without HT + Tyr (10 µM each). Beta-actin was used for normalization. Total caspase-3 is shown as control of loading. Caspase-3 activity is expressed as arbitrary density unit (ADU). ^b p < 0.01 vs. 1% FCS, ^d p < 0.01 versus H₂O₂ 100 µM. The gels shown are representative of three runs with similar results.