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. 2017 May 16;2(2):139–155. doi: 10.1002/btm2.10063

Figure 4.

Figure 4

Polymer degradation and molecular weight influence DC activation in cell culture and mice. (a) PBAEs formulated with 4 (PBAE‐4, blue), 6 (PBAE‐6, red), or 10 (PBAE‐10, green) carbons in the diacrylate monomer backbone were synthesized with (b) different starting molecular weights, but similar degradation profiles. (c) PBAEs were degraded to distinct molecular weight ranges, formulated into particles, and used to treat DCs. Maximum activation, as indicated by CD40 expression on live (DAPI−) DCs, correlated with a molecular weight range of 1,500–3,000 Da. (d) After introduction into mice, immunogenic PBAE particles (red) induced statistically significant activation of lymph node resident immune cells compared to treatment with soluble (blue, “Free”) or buffer control (gray, “Vehicle”) treatments. (a–c) adapted with permission from Andorko JI, Pineault KG, Jewell CM. Impact of molecular weight on the intrinsic immunogenic activity of poly(beta amino esters). J Biomed Mater Res A. 2017;105(4):1219‐1229. (d) Reprinted with permission from Andorko JI, Hess KL, Pineault KG, Jewell CM. Intrinsic immunogenicity of rapidly‐degradable polymers evolves during degradation. Acta Biomater. 2016;32:24‐34