Skip to main content
. 2001 Jul 15;29(14):2938–2949. doi: 10.1093/nar/29.14.2938

Figure 1.

Figure 1

nop12-1 is synthetically lethal with nop2-4. (A) Diagram of strain YJPA24 used for the sl screen. Retention of pJPA84 is selected on minimal medium minus tryptophan. Spontaneous loss of pPW67 at permissive temperature (25°C) results in white sectors in red colonies and confers resistance to 5-FOA. (B) Growth at 25°C on medium containing 5-FOA. YJPA22 was transformed with pRS314, or plasmids bearing NOP2 or different nop2 ts alleles. NOP2 supports loss of pPW67, whereas pRS314 does not. Temperature sensitive nop2 alleles support spontaneous loss of pPW67 and growth to different extents. (C) Allele specificity. The nop12-1 allele in YKW35 is synthetically lethal with nop2-4, nop2-9 and nop2-10, but not with nop2-3, nop2-5 or nop2-6. YKW35 transformants that carry nop2-4, nop2-9 or nop2-10 cannot spontaneously lose pPW67 and remain viable. (D) Complementation of synthetic lethality. Region of chromosome XV that complements the sl phenotype in YKW157. Open arrows denote endpoints of insert in pKW28. Filled arrows denote insert in pKW30. RPS15 is also known as RPS21. (E) NOP12 supports growth on medium containing 5-FOA. YKW35 bearing nop2-4 (on pJPA30) can afford to lose pPW67 and remain viable on medium containing 5-FOA if transformed with NOP2 (pBH49) or NOP12 (pKW39), but not with control plasmid pRS313, or plasmids bearing RPS15 (pKW36) or RPP2A (pKW38).