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NER in Polβ mutant extracts. In vitro NER of AAF-adducted DNA was performed in yeast cell-free extracts of the wild-type (WT) strain TC102 (lane 1) and the Polβ (pol4) deletion mutant strain SK-2-1β (lane 2). Top, ethidium bromide-stained gel; bottom, autoradiograph of the gel. +AAF, damaged pUC18 DNA; –AAF, undamaged pGEM3Zf DNA as the internal control.
