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. 2001 Jul 15;29(14):3123–3130. doi: 10.1093/nar/29.14.3123

Figure 3.

Figure 3

BER in Polβ mutant extracts. (A) In vitro BER of OsO4-damaged DNA was performed in yeast cell-free extracts of the wild-type (WT) strain TC102 (lane 1) and the Polβ deletion mutant (pol4) strain SK-2-1β (lane 2). +OsO4, damaged pUC18 DNA; –OsO4, undamaged pGEM3Zf DNA as the internal control. Top, ethidium bromide-stained gel; bottom, autoradiograph of the gel. (B) In vitro BER of the uracil-containing 30mer duplex DNA was performed in yeast cell-free extracts of the wild-type (WT) strains SX46A (lane 1) and TC102 (lane 2), or in yeast cell-free extracts of the Polβ deletion mutant (pol4) strain SK-2-1β (lane 3). Repair products were separated by electrophoresis on a 20% denaturing polyacrylamide gel and visualized by autoradiography. DNA size markers in nucleotides are indicated on the right.