Fig. 2.

HDAC inhibitor treatment rapidly induces CD1 D mRNA and protein. a JeKo-1 cells were treated with 1 µM TSA for 0, 1, 2, 3, and 4 h, and CD1 D mRNA levels were assessed by RT-PCR. 18S serves as the loading control. Fold change in CD1D mRNA is calculated as CD1D/18S. The lower panel shows a graphical representation of CD1D/18S fold change versus time. The data are representative of three independent experiments. b LCD1d were treated with 1 µM TSA for 4 h, and cell surface expression of CD1d was assessed by flow cytometry. Vehicle-treated cells are represented by the shaded gray histogram. TSA-treated cells are represented by the red histogram. c LCD1d-DR4 was treated with 1 µM TSA for 4 h, and cell surface expression of MHC class II was determined by flow cytometry. d, e CD1d cell surface levels of vehicle- and TSA-treated JeKo-1 (d) and SP53 (e) were assessed by flow cytometry. Geometric means (GM) were calculated and are representative of the changes seen in three independent experiments