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. 2017 Sep 1;8:69. doi: 10.1186/s40104-017-0201-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Sequential purification steps of recombinant OVGP1 (R_OVGP1). Chromatogram of (a) IMAC based affinity purification: purification of recombinant OVGP1 through IMAC resulted in a single peak (indicated by arrow) after applying a gradient of 0–100% (300 mmol/L imidazole) in 30 mL. The peak fractions contained partially purified recombinant OVGP1. b Ion-Exchange Chromatography: Purification step through IEX showing elution of three merged peaks (indicated by arrows) during a stepwise gradient run. The latter peak contained recombinant OVGP1 with a few remaining contaminating proteins. c Size-Exclusion Chromatography: Purification of recombinant OVGP1 through the step resulted in elution of a peak (indicated by arrow) containing purified recombinant OVGP1