Fig. 8.

A PCR product derived from FanAAMT-adjacent sequence segregates with the potential to produce MA. Panel a shows the position of the primers relative to the gene. Panel b shows the presence or absence of the associated amplicon in the ‘Mara des Bois’ and ‘Elyana’ parental lines and twelve segregating progeny. A co-amplifying product (shown below) serves as a control for DNA quality, and is reproducibly faint in the ‘Elyana’ parent and some progeny. The same fruits were tested for the presence of the transcript and volatile, demonstrating that the transcript segregates 1:1 with the marker, and is necessary, yet not sufficient, for high-amplitude production of MA