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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Cell Rep. 2017 Jun 27;19(13):2657–2664. doi: 10.1016/j.celrep.2017.05.092

Figure 4. PpV synergizes with Fmt in vivo.

Figure 4

(A–G) Light micrographs of Drosophila adult eyes are shown. Removing one copy of fmt or PpV slightly enhances GMR>Egr small eye, while simultaneously remove one copy of each results in complete eye loss. Co-expression of Fmt and PpV synergistically suppress GMR>Egr eye phenotype.

(H–K) Light micrographs of Drosophila adult wings are shown. Compared with controls (H), loss of either Fmt (I) or PpV (J) driven by nub-Gal4 produced no obvious phenotype, while co-expression of Fmt-IR and PpV-IR resulted in smaller wings (K). (L) Quantification data of wing size in A–D. (M–P) Fluorescence micrographs of GFP-labeled clones of eye-antennal disc and VNC are shown. lgl−/−/RasV12 induced tumor overgrowth (M′), invasion (M″), and eventually death as giant larvae (M). Ectopic expression of Fmt partially restored the pupation (N), significantly suppressed tumor invasion (N″), but not tumor growth (N′). Expression of PpV alone is sufficient to completely suppressed invasive phenotype (O″) and dramatically restored pupation (O), whereas tumor size was only slightly suppressed, if there is any (O′). Co-expression of Fmt and PpV dramatically suppressed tumor progression, including tumorigenesis and invasion (P′ and P″), and rescued all the animals to pupal stage (P). % indicates phenotype penetrance ratio. (Q) Model of PpV and Fmt in RasV12-induced tumorigenesis. Scale bars, 400 μm in (M–P), 200 μm in (M′–P′), 100 μm in (M″–P″). Genotypes: (A) UAS-Egr/+; GMR-Gal4/+ (B) UAS-Egr/+; GMR-Gal4/fmt1, FRT79E (C) PpVΔ1, FRT19A/+; UAS-Egr/+; GMR-Gal4/+ (D) PpVΔ1, FRT19A/+; UAS-Egr/+; GMR-Gal4/fmt1, FRT79E (E) UAS-Egr/UAS-Fmt; GMR-Gal4/+ (F) UAS-Egr/+; GMR-Gal4/UAS-PpV (G) UAS-Egr/UAS-Fmt; GMR-Gal4/UAS-PpV (H) nub-Gal4/+ (I) nub-Gal4/UAS-Fmt-IR (J) nub-Gal4/+; UAS-PpV-IR/+ (K) nub-Gal4/UAS-Fmt-IR; UAS-PpV-IR/+ (M) ey-Flp1/+; tub-Gal80, FRT40A/lgl4, FRT40A, UAS-RasV12; Act>y+>Gal4, UAS-GFP/+ (N) ey-Flp1/+; tub-Gal80, FRT40A/lgl4, FRT40A, UAS-RasV12; Act>y+>Gal4, UAS-GFP/UAS-Fmt (O) ey-Flp1/+; tub-Gal80, FRT40A/lgl4, FRT40A, UAS-RasV12; Act>y+>Gal4, UAS-GFP/UAS-PpV (P) ey-Flp1/+; tub-Gal80, FRT40A/lgl4, FRT40A, UAS-RasV12; Act>y+>Gal4, UAS-GFP/UAS-Fmt, UAS-PpV. See also Figure S4.