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Detection of known extracellular vesicle-derived miRNA by reverse-transcription quantitative PCR in pregnant and embryonic-mortality cow. Four miRNAs identified by deep sequencing were tested at Day 17 of gestation. Three miRNAs (miR-16b, -25, and -3596) were significantly different in relative abundance between embryonic-mortality and pregnant cows (P<0.05); one (miR-100) was not validated by reverse-transcription quantitative PCR (data not shown).
