Fig. 1.

Myeloid-derived suppressor cell subsets identified in human spleen tissue. a Human splenocytes or PBMC from the same donor were stained with antibodies specific for Lineage markers (CD3, CD19, and CD56), CD11b, and CD33. The frequency of total MDSCs (Lin−, CD11b+, CD33+, HLA-DR−), CD15 + MDSCs (Lin−, CD11b+, CD33+, HLA-DR−, CD15+, CD14−), and CD14 + MDSCs (Lin−, CD11b+, CD33+, HLA-DR−, CD15−, CD14+) was determined by flow cytometry. b The frequency of MDSCs subsets in total PBMC or splenocytes was compared using ANOVA and compared between PBMC and spleen samples using a paired t test (c)