Table 2.
Mass spectrometry summary of RSV purification methods
| Origin | Protein Name (Uniprot) | Sucrose-Purified RSV | RSV-Conditioned Media | FPLC | FPLC❖ | Peak FPLC◆ | Tail FPLC◆ | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PSM | CVG | PSM | CVG | PSM | CVG | PSM | CVG | PSM | CVG | PSM | CVG | ||
| Bovine | Serum Albumin | 13 | 22% | 349 | 68% | 69 | 45% | 33 | 40% | 32 | 39% | 34 | 44% |
| RSV | Nucleo-protein | 18 | 27% | ND | ND | 15 | 32% | 7 | 25% | 2 | 8% | 2 | 7% |
| Human | Histone H4 | 5 | 41% | ND | ND | 2 | 21% | 2 | 21% | ND | ND | 2 | 21% |
| Human | GAPDH | 4 | 18% | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
PSM = peptide spectral matches; CVG = % coverage of the observed proteins; ND = not detected.