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Effect of read length on de novo assembly metrics. De novo assemblies were generated using simulated reads of four different lengths (50, 100, 250 and 500 bp) and evaluated using QUAST. The effect of read length is shown for: (a) N50, (b) the largest contig, (c) the total number of contigs, and (d) the total length of the assembly. After 250 bp, the gain from longer read lengths levels off.
