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. 2017 Aug 21;9(8):234. doi: 10.3390/v9080234

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Intranasal immunization with adenovirus-vectored influenza vaccine induced long-lived HA stalk-specific humoral immunity. To detect long-term stalk-specific antibody responses, Balb/c mice were intranasally immunized with FiPR8+CT, live PR8, or rAdH5/M2e. Sera were collected from immunized mice at twelve moths post-immunization, and the levels of Th1/Th2 Abs were determined by a Mouse immunoglobulin Isotyping Magnetic Bead Panel assay (Millipore, Millipore Corporation, Billerica, MA, USA) (B–E). To examine the duration of the vaccine-specific and stalk-specific antibody responses, sera were collected from the immunized mice at three, nine, or twelve months post-immunization. The levels of vaccine-specific and stalk-specific Abs were determined by ELISA using plates coated with H1N1 (A), or baculorvirus-expressed cH9/1 protein (F). The values represent the mean ± SEM (vertical bars) end point ELISA antibody titers determined from five mice per group (*** p < 0.0001).

Intranasal immunization with adenovirus-vectored influenza vaccine induced long-lived HA stalk-specific humoral immunity. To detect long-term stalk-specific antibody responses, Balb/c mice were intranasally immunized with FiPR8+CT, live PR8, or rAdH5/M2e. Sera were collected from immunized mice at twelve moths post-immunization, and the levels of Th1/Th2 Abs were determined by a Mouse immunoglobulin Isotyping Magnetic Bead Panel assay (Millipore, Millipore Corporation, Billerica, MA, USA) (B–E). To examine the duration of the vaccine-specific and stalk-specific antibody responses, sera were collected from the immunized mice at three, nine, or twelve months post-immunization. The levels of vaccine-specific and stalk-specific Abs were determined by ELISA using plates coated with H1N1 (A), or baculorvirus-expressed cH9/1 protein (F). The values represent the mean ± SEM (vertical bars) end point ELISA antibody titers determined from five mice per group (*** p < 0.0001).