| Data collection |
|
| Electron energy (kV) |
200 |
| Electron dose (e-/Å2) |
50 |
| Pixel size corresponding to the physical detector sensor (Å) |
0.98 |
| Defocus range (μm) |
-3.0 to -1.0 |
| Number of micrographs |
16,111 |
|
| Structure determination of the non-AAA subcomplex |
|
| Cryo-EM refinement |
| Number of particles used in refinement |
117,471 |
| Final Resolution of RP complex (Å) |
4.5 |
| Isotropic B-factor of RP complex (Å2) |
-90 |
| Pseudo-crystallographic refinement of atomic model |
| Cell dimension a, b, c(Å) |
250.9,250.9,250.9 |
| Cell angle α, β, γ (Å) |
90,90,90 |
| Space group |
P1 |
| Resolution range |
250.88-4.5 |
| Number of reflections |
652,468 |
| Number of atoms |
36,739 |
| Rwork factor (%) |
31.2 |
| Geometric Parameters (RMSD) |
| Bond length (Å) |
0.0043 |
| Bond angle (°) |
0.88 |
| Ramachandran plot statistics |
| Preferred (%) |
91.0 |
| Allowed (%) |
8.9 |
| Outlier (%) |
0.13 |
| MolProbity validation |
| Rotamer outlier (%) |
0.37 |
| MolProbity score |
2.3 |
| Clashscore |
15.78 |
| C-beta outliers |
0 |
|
| Conformational states of the p28-bound RP |
|
| State |
Particle number |
Resolution (Å) |
B-factor (Å2) |
Ramachandran plot (%) |
Rotamer outlier (%) |
|
|
|
|
Favored |
Allowed |
Outlier |
|
| T1 |
39,520 |
5.7 |
-80 |
87.8 |
12.0 |
0.27 |
0.99 |
| T2 |
27,629 |
6.1 |
-120 |
87.4 |
12.4 |
0.28 |
1.21 |
| T3 |
10,891 |
6.8 |
-120 |
88.9 |
11.0 |
0.11 |
0.52 |
| TA1 |
12,223 |
8.5 |
-350 |
81.2 |
18.1 |
0.67 |
0.79 |
| TA2 |
11,082 |
8.3 |
-400 |
79.5 |
19.9 |
0.61 |
2.1 |
| TA3 |
21,031 |
7.3 |
-350 |
83.6 |
16.0 |
0.41 |
0.72 |
| TA4 |
9,004 |
8.3 |
-400 |
79.6 |
19.7 |
0.7 |
1.15 |
| TA5 |
11,767 |
7.9 |
-450 |
83.0 |
16.5 |
0.48 |
0.62 |
| TA6 |
11,610 |
8.9 |
-450 |
82.3 |
17.2 |
0.46 |
0.67 |
| TA7 |
14,464 |
7.7 |
-450 |
82.0 |
17.6 |
0.36 |
1.05 |
