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. 2001 Jul 15;29(14):2950–2962. doi: 10.1093/nar/29.14.2950

Figure 6.

Figure 6

Figure 6

Mitochondrial localization of gBP29 and gBP27. (A) Total cell extracts of C.fasciculata were submitted to renografin gradient centrifugation (20–35% w/v) and 36 fractions were collected (fraction 1 was taken from the top of the gradient, etc.). Protein extracts from indicated fractions were submitted to SDS–PAGE (10% polyacrylamide), followed by western blotting with antisera against gBP29 and gBP27 (upper two panels) and mt ATPase and GAPDH (lower two panels). The same fractions were tested for the presence of mitochondrial 9S and 12S rRNA by northern blot hybridization (middle panel). (B) Helical wheel projection of the putative mitochondrial import signal of gBP29 (left) and gBP27 (right). Charged and hydrophilic amino acids are boxed.