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. 2001 Jul 15;29(14):2950–2962. doi: 10.1093/nar/29.14.2950

Figure 7.

Figure 7

Fractionation of mt extract on a Sephacryl S-500 gel filtration column. Mitochondrial extracts were fractionated on a Sephacryl S-500 column as described in Materials and Methods. Fractions were tested by northern blot hybridisation (upper) for the presence of ND7 mRNA (dotted line; A.U. = arbitrary units), the combined presence of ND7[FS], ND7[5′], Cyb-II and MURF2-II gRNAs (solid line) and for 9S + 12S rRNA (inserted bar; the elution profile of 9S + 12S rRNA is indicated by shading). The protein concentration of every fifth fraction was determined by Bradford assay (dashed line). Marker proteins used to calibrate the Sephacryl column were: thyroglobulin (647 kDa), apoferritin (443 kDa) and cytochrome c (12.3 kDa). Every fifth fraction was tested for the presence of gBP29 and gBP27 by western blotting (panels 2 and 3 from the top), for RNA ligase activity by self-adenylylation of two proteins of 43 and 49 kDa, respectively (panel 4), and for TUTase activity by uridylate addition to tRNAs (bottom). Mt, mitochondrial extract.