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. 2017 Aug 23;23:4067–4076. doi: 10.12659/MSM.902806

Figure 3.

Figure 3

We investigated the relationship between ROS generation, caspase-3 and caspase-9 expression, HO-1 induction, and Akt/ILK, Nrf2, and JNK signaling pathways. (A, B) After treatment with siRNAs of ILK, HO-1 and Nrf-2, ROS level and caspase-9/3 expressions were assessed. The data were analyzed by independent-samples t test. (C, D) After podocyte synchronization, cells were treated with 10 uM SnPPIX for 1 h, 20 uM SP600125 for 0.5 h, and 50 uM LY294002 for 1 h. ROS level and caspase-3 and caspase-9 expressions in podocytes were evaluated. The data were analyzed by independent-samples t test. * Compared with control, # compared with high glucose group, & compared with high glucose+salidroside group P<0.05. Sn (HO-1 inhibitor SnPPIX), SP (JNK inhibitor SP600125), LY (ILK inhibitor LY294002). At least 3 independent experiments with 3 replicates per experiment were conducted.