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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Hypertens. 2016 Jan;34(1):68–78. doi: 10.1097/HJH.0000000000000760

FIGURE 2.

FIGURE 2

Comparable expression of CHGA in wild-type Gly/Gly and variant Ser/Ser transgenic mouse models: (a) Similar transcription of the CHGAGly364 and CHGASer364 transgenes was observed in the humanized mouse models. Expression of the CHGA transgene was quantified by RT-PCR assay using human CHGA gene specific primers. The mRNA expression was normalized against 18S RNA. Error bars indicate SEM. (b) Western blot analysis of CHGA expression: total protein extracts of adrenal (3 μg) and brain (20 μg) tissue of the homozygous Gly/Gly and Ser/Ser mouse models were probed for expression levels of CHGA protein. The blots were stripped and probed with housekeeping protein GAPDH or actin. Both the allelic variants of CHGA express the transgene protein at comparable levels.