Figure 4.

Relative expression of genes involved in flavonoid biosynthesis in leaves of nonmycorrhized M. truncatula plants in response to 100 μm ABA and 1 mm MeJA treatments. Total RNA was isolated after 24 h of treatment, and changes in transcript abundance were determined by quantitative RT-PCR analysis. The relative expression of MtATHB-7 (MTR_8g026960), MtMYC2 (MTR_8g067280), MtAOS (MTR_4g068550), MtJAZ (MTR_5g013530), MtLDOX (MTR_3g070860), Mt3′GT (MTR_5g016660), MtUGT73K1 (MTR_4g031800), and MtCHMT (MTR_7g011990) genes was calculated as 2^−ΔCq and normalized using MtTef-1a as an internal reference gene. Bars represent means ± sd from six plants. Asterisks indicate significant differences between treatments and the control (one-way ANOVA, P < 0.05 [*], P < 0.01 [**], and P < 0.001 [***]; GraphPad Prism).