Table III. List of metabolites increased or decreased in leaves of AM- and Pi-treated plants compared with controls.
| m/z | Retention Time | Metabolite Class | AM Versus Control | Q | Pi Versus Control | Q |
|---|---|---|---|---|---|---|
| min | ||||||
| Flavonoids | ||||||
| 355.1 | 1.848 | Prenylchalcone | −1.89 | 0.000 | −1.17 | 0.442 |
| 353.1 | 1.909 | Prenylchalcone | −1.93 | 0.000 | −1.21 | 0.383 |
| 613.2 | 1.249 | Proanthodelphinidin | 2.09 | 0.027 | 1.35 | 0.725 |
| 575.2 | 3.030 | Fromosin-7-O-glucoside-6′′-O-malonate | 1.35 | 0.026 | 1.20 | 0.554 |
| 575.2 | 3.181 | Fromosin-7-O-glucoside-6′′-O-malonate | 1.23 | 0.027 | 1.16 | 0.561 |
| 649.3 | 3.394 | Apigenin glucuronic glucoside | −3.23 | 0.026 | −2.41 | 0.205 |
| 991.2 | 3.598 | Luteolin glucuronic 5OH-coniferyl alcohol | 1.59 | 0.028 | 1.37 | 0.776 |
| Other phenylpropanoids | ||||||
| 344.1 | 2.702 | Coumaroyl-glucoside | 1.55 | 0.028 | 1.58 | 0.117 |
| 439.2 | 2.797 | 1′-O-Benzyl-l-rhamnopyranosyl | 1.35 | 0.039 | 1.12 | 0.762 |
| 439.2 | 3.050 | 1′-O-Benzyl-l-rhamnopyranosyl | −1.53 | 0.026 | −1.18 | 0.115 |
| 476.2 | 3.450 | N-Feruloyltyramine glucoside | −1.59 | 0.008 | −1.15 | 0.130 |
| 353.1 | 3.174 | Ferulic acid-GlcA | −0.70 | 0.011 | −1.71 | 0.012 |
| 445.1 | 3.174 | Ferulic acid-GlyA-glycerol | −1.69 | 0.011 | −1.74 | 0.012 |
| 314.1 | 3.451 | N-Feruloyltyramine | −1.57 | 0.022 | −1.17 | 0.113 |
| 314.1 | 3.940 | N-Feruloyltyramine | −1.70 | 0.009 | −1.17 | 0.352 |
| 561.2 | 3.170 | Dilignol glucoside | 1.21 | 0.020 | 1.33 | 0.227 |
| Carotenoid derivatives and lipids | ||||||
| 433.2 | 4.298 | Dihydroxy blumenol glucoside | −30,090 | 0.000 | 1.28 | 0.332 |
| 465.2 | 4.198 | Blumenol B malonylglucoside | −2.17 | 0.001 | −1.97 | 0.101 |
| 285.2 | 3.566 | Dihydroxyphaseic acid-like | −3.07 | 0.028 | −2.30 | 0.117 |
| 487.2 | 3.479 | Tributyrin glucoside (triacylglycerol) | −3.11 | 0.028 | −1.95 | 0.131 |
| 537.3 | 6.021 | MGMG (18:3) | 1.97 | 0.027 | 1.79 | 0.306 |
| 799.5 | 7.715 | MGDG (36:5) | 1.40 | 0.042 | 1.38 | 0.401 |
| Saponins | ||||||
| 1383.6 | 4.420 | Medicagenic acid derived | 1.29 | 0.044 | 1.43 | 0.115 |
| 1385.6 | 4.088 | Zhanic acid derived | 1.38 | 0.031 | 1.23 | 0.612 |
| 1564.7 | 4.098 | 3-Glc-Glc-Glc, 23-Ara, 28-Ara-Rha-Xyl zhanic acid | 1.73 | 0.005 | 1.33 | 0.621 |
| 1254.6 | 4.192 | Hederagenin derived | −1.37 | 0.009 | 1.04 | 0.630 |
| 953.5 | 4.225 | Oleanolic acid derived | −1.48 | 0.014 | −1.05 | 0.437 |
| 957.5 | 4.690 | dHex-Hex-HexA-Hederagenin | −2.13 | 0.011 | −1.49 | 0.101 |
| 1117.5 | 4.732 | Hederagenin derived | −2.27 | 0.022 | −1.55 | 0.323 |
| 999.5 | 5.200 | Soya_B derived | −2.46 | 0.024 | −1.54 | 0.115 |
| 1029.5 | 5.202 | Soya_B derived | −2.24 | 0.045 | −1.47 | 0.115 |
| 867.5 | 5.203 | Soya_B derived | −2.54 | 0.025 | −1.66 | 0.115 |
| 1011.5 | 5.218 | Soya_B derived | −2.12 | 0.037 | −1.48 | 0.118 |
| 1029.5 | 5.447 | Soya_B derived | −2.37 | 0.041 | −1.69 | 0.115 |
| 1131.6 | 5.235 | Sophoradiol derived | −2.34 | 0.032 | −1.45 | 0.182 |
| Glucoside conjugates | ||||||
| 342.1 | 1.599 | 6-(α-d-Glucosaminyl)-1D-myoinositol | 1.19 | 0.032 | 1.05 | 0.721 |
| 289.2 | 3.747 | 1-O-Hexyl-d-glucitol | 1.23 | 0.006 | 1.33 | 0.127 |
| 361.1 | 1.821 | Syringaldazine | 1.39 | 0.020 | 1.21 | 0.700 |
| 267.2 | 3.116 | 1-O-Hexyl-d-Mannitol | −1.26 | 0.028 | 1.00 | 0.628 |
| 223.2 | 2.916 | 2-Benzyl-2-hydroxybutanedioate | −1.38 | 0.000 | 1.10 | 0.428 |
| 317.1 | 2.676 | Glc-l-rhodinose | −1.36 | 0.014 | −1.28 | 0.205 |
| 207.1 | 3.181 | 2-Benzylsuccinate | −1.72 | 0.089 | −1.36 | 0.023 |
Values marked in boldface represent significant alterations presented as metabolite ratios in AM or Pi plants versus controls (Q < 0.05). Positive and negative ratios indicate increased and decreased levels, respectively. The values presented are based on peak area normalized against leaf fresh weight and are means of seven to eight plants. m/z is the mass-to-charge ratio of the molecular ion. Complete data sets of metabolites are presented in Supplemental Tables S8 and S9.