Figure 2.

Purified BcXYG1 induces necrosis in multiple plants. BcXYG1 and control proteins fused to 6× His were expressed in E. coli, purified, suspended in phosphate-buffered saline (PBS), and infiltrated into leaves. A, Immunoblot analysis of proteins expressed in E. coli using anti-His antibodies. EGFP-6×His, EGFP (negative control); BcXYG1-6×His, native BcXYG1; MBcXYG1-6×His, mutant BcXYG1 that lacks enzymatic activity; BcXYG2-6×His, BcXYG1 homolog that lacks necrosis-inducing activity. B, Response of N. benthamiana leaves to different concentrations of BcXYG1-6×His. C, Treatment of N. benthamiana leaves with 100 μg mL⁻¹ purified proteins. D, Treatment of bean, tomato, wheat, and maize leaves with 100 μg mL⁻¹ purified proteins. Images in B to D were taken 5 d after treatment.