Figure 6.

BcXYG1 induces resistance in bean. A, One of the first two true leaves of a 9-d-old bean seedling was infiltrated with 500 μL of purified protein solution. The plants were kept in a growth chamber for 48 h, the second leaf was then inoculated with wild-type B. cinerea spores, and the plants were kept in a humid environment. Photographs were taken (left) and average lesion size was determined (right) 72 hpi. Data represent means and sd from three independent experiments, each with six replications. Different letters in the graph indicate statistical differences at P ≤ 0.01 using one-way ANOVA. B, Untreated leaves were picked 48 h after treatment, and relative expression levels of the defense genes Pvd1, PvPR1, and PvPR2 were determined by qRT-PCR analysis. The expression level in blank control plants was set as 1. The expression level of the bean Actin-11 gene was used to normalize different samples. Data represent means and sd of three independent replicates.