Figure 5.

Microfibrils contain 1 to 4 linked Glc. In vitro products were pretreated with 2% SDS and proteinase K, followed by α-amylase and amyloglucosidase to remove P. pastoris-derived α-1,4-glucan chains. Thereafter, the sample was permethylated to alditol acetates, and then subjected to GC/MS analysis. The resulting spectra are compared with reference peaks. Identities of each peak are marked. In particular, terminal-Glc and 4-linked Glc were detected at 9.348 min and 19.059 min, respectively. t-Glc, terminal-Glc; 4-Glc, 4-linked Glc.