Induction of NST1 and NST2 expression by MYB26. A, NST1Pro:GUS expression in wild type and (B) increased NST1pro:GUS expression in MYB26 overexpression line, particularly in the peduncle, sepals. and anthers. C, Increased magnification of NST1pro:GUS expression in MYB26 overexpression line showing expression in anthers. D and E, Time course analysis of expression of NST1 and NST2 by qRT-PCR after DEX activation of MYB26 in the transgenic (MYB26pro:MYB26-GR-YFP) myb26 mutant line and in the myb26 mutant control lacking the transgene. D, Induction of NST1 occurred 4 to 6 h after DEX treatment. E, Induction of NST2 was seen 4 to 6 h after DEX treatment. Error bars represent sd (t test statistical analysis compared to 0 h in each line; *P ≤ 0.05; **P ≤ 0.01).