Figure 3.

Nodule development in wild-type cv Parvus (WT) and SL-deficient ccd8-1 plants infected with lacZ-labeled R. leguminosarum bv viciae. A, Number of infection threads per cm of root (four to 10 root segments from n = 6–11 plants) in two independent experiments. B, Photograph of stained roots showing infection threads in wild-type and ccd8-1 plants. Bars = 0.1 mm. C, Photograph of stained root showing nodules in wild-type and ccd8-1 plants. Bar = 5 mm. D, Number of developing nodules per cm of root (10 root segments from n = 15 plants). E, Ethylene evolution per gram nodule dry weight (DW) from the acetylene reductase assay (n = 4). Values are means ± se, and values statistically different from the wild type are indicated by asterisks: *, P < 0.05. In B and C, R. leguminosarum is stained blue.