Figure 10.

Immunodetection and immunoprecipitation of Salicornia and Sarcocornia OAS-TL proteins. A and B, Immunodetection of OAS-TL A and OAS-TL B, respectively. Fifty µg proteins of Salicornia and Sarcocornia were subjected to in-gel Cys desulfhydrase activity as shown in Fig. 9B. The activity bands were then sliced and were fractionated by 12.5% SDS PAGE together with Arabidopsis wild-type (Col) crude extract and analyzed by Western blot with antibodies raised against Arabidopsis OAS-TL A or OAS-TL B. C and D, Immunoprecipitation of 30 µg Salicornia and Sarcocornia desulfhydrase activity employing antibodies raised against Arabidopsis OAS-TL A and OAS-TL B, respectively. Plus sign (+) indicates “with antibody”; (−) minus sign indicates “without antibody”; red pointer indicates activity band.