Figure 4.

Leaf morphology and ploidy of IL2-5/IL4-3 double homozygote plants. A, Representative propidium iodide-stained leaflet cross sections (left) and thickness measurements (right) for the seventh leaf of greenhouse-grown M82 and double homozygous IL2-5/IL4-3 plants (n = 10). **, P < 0.01. Bars = 200 μm. B, Circularity (ratio of area to the square of the perimeter) of distal lateral leaflets as in A. Silhouettes of representative M82 and IL2-5/IL4-3 leaflets are shown above the bars. Letters indicate statistical significance in each pairwise genotype comparison (P < 0.05). C, Distribution of relative nuclear sizes reflecting endoreduplication in leaflets as in A and B (n = 5). Letters denote statistical significance between pairwise genotype comparisons at each ploidy level. Sp, S. pennellii. D, Leaf plastochron P3 dimensions calculated from 3D surface reconstructions of vegetative shoot apices (n = 9; *, P < 0.05 and **, P < 0.01 relative to M82).