Figure 6.

SCYL2B is localized at the Golgi, TGN, and PVC. A, YFP:SCYL2B is tip localized in growing root hairs. B, The tip-localization pattern disappears in mature root hairs. Root hairs of transgenic Arabidopsis plants expressing YFP:SCYL2B were observed by wide-field fluorescence microscopy. Bars in A and B = 10 μm. C to E, Localization of SCYL2B in various subcellular organelles. Arabidopsis leaf protoplasts were cotransformed with YFP:SCYL2B and the indicated constructs, which encode protein markers for the Golgi (C), TGN (D), and PVC (E). The localization of SCYL2B was examined by the detection of YFP, CFP, and RFP signal by confocal microscopy. White color indicates strong colocalization. Arrows indicate spots showing colocalization. Bars = 20 μm.