Figure 9.

CSLD3 is mislocalized in root hairs of scyl2b mutants. Localization of proteins that are known to function at the root hair tip was observed in the background of Col-0 and scyl2b-3 mutants. The expression of N-terminal GFP fusion proteins of ROP2, RHD2, RABA4B, SYP123, PRP3, and CSLD3 was driven by the EXPA7 promoter, a root hair-specific promoter. For each gene construct, at least five independent T2 transgenic plants were used in two independent experiments that yielded similar results. Root hairs of transgenic plants were observed by confocal microscopy. White arrows indicate the difference of GFP:CSLD3 localization. Bars = 10 μm.