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. 2017 Aug 2;175(1):210–222. doi: 10.1104/pp.17.00885

Figure 2.

Figure 2.

Quantitative analysis of S4B fluorescence intensity in leaf midribs of P. patens wild type (WT), ppcesaKO, and rescue lines. Genotypes with different letters are significantly different (P < 0.05). A, Fluorescence was significantly weaker in ppcesa3/8KOs compared with the wild type. ppcesa3KOs were not significantly different from the wild type, whereas ppcesa8KOs were intermediate between the wild type and ppcesa3/8KOs and significantly different from both. For each mutant genotype, three independent genetic lines were sampled in triplicate. Two independent wild type lines (GD06 and GD11) were sampled in triplicate. Error bars indicate se for three mutant (n = 3) or two wild type (n = 2) lines. B, Lines derived from transformation of ppcesa3/8KO-86lox with proCESA8::CESA8 (8R) had significantly higher fluorescence compared with the parent double KO line and ppcesa8KO but significantly less than the wild type. C, Lines derived from transformation of ppcesa3/8-86lox with proCESA3::CESA3 (3R) had significantly higher fluorescence compared with the parent double KO line (except 3R29) and were not significantly different from either ppcesa8KO lines (3R29 and 3R52) or the wild type (3R45). For B and C, error bars indicate se for explants from the same line (n = 3 or n = 2 [WT, 3/8KO, and 8KO in C]).