Figure 6.
Reverse transcription quantitative PCR analysis of PpCESA3 and PpCESA8 expression in the wild type, ppcesa3KOs, and ppcesa8KOs. Target/average reference cross-point ratios (using actin and v-type H+-translocating pyrophosphatase reference genes) were determined for three independent lines of each mutant (3KO-5, 3KO-35, 3KO-126; 8KO-5B, 8KO-4C, 8KO-10C; and 3/8KO-43, 3/8KO-57, 3/8KO-86) and two independent wild type lines (GD06 and GD11) with two technical replicates each. Error bars indicate se for the three mutant (n = 3) or two wild type (n = 2) lines.