Figure 4.

Effect of different fractions of OHS extract on NO production (A) and iNOS expression (B) in RAW264.7 cells. Normal, untreated cells; Control, addition of 1 µg/ml LPS only; Hex, addition of 1 µg/ml LPS plus hexane fraction at 100 µg/ml. EA, addition of 1 µg/ml LPS plus ethylacetate fraction at 100 µg/ml. n-BuOH, addition of 1 µg/ml LPS plus butanol fraction at 100 µg/ml. H2O, addition of 1 µg/ml LPS plus water fraction at 100 µg/ml. EtOAc, butanol, and water fractions were dissolved in water and the hexane fraction was first dissolved in DMSO and then diluted with water. Data are representative of three independent experiments (mean and SEM). One-way ANOVA. *p<0.05; ***p<0.001; ****p<0.0001; ns, not statistically significant