Fig. 4. Ab initio SAXS reconstruction of Tim50^IMS.

(A) Tim50^IMS SAXS molecular envelope aligned with the homology model. The ab initio envelope generated by DAMAVER analysis of SAXS data from Tim50^IMS (0.5 mg/ml; gray) is shown in comparison with the Tim50^IMS homology model (ribbon structure), aligned using SITUS. The domains of the Tim50^IMS homology model are color-coded with respect to the linear domain representation shown above. (B) Analysis of the fit between the envelope and the homology model. The experimental SAXS scattering data for Tim50^IMS (0.5 mg/ml; black) and theoretical scattering data for the Tim50^IMS homology model (red) were fit using the CRYSOL package, yielding a χ² value of 1.12.