Skip to main content
. 2017 Sep 1;3(9):e1700532. doi: 10.1126/sciadv.1700532

Fig. 8. Substrate-dependent changes in the Tim50-Tim23 interaction with and without CL.

Fig. 8

Analysis of in organello cross-linking between Tim50 and [35S]Tim23(S80C) (left) or between Tim50 and [35S]Tim23(F114C) (right) was conducted as above (Fig. 2), except in the presence of increasing pSu9-DHFR concentration. Panels above each graph show a representative cross-linked adduct between Tim50 and the relevant [35S]Tim23 construct (closed arrowhead). Relative cross-linking efficiency means represent relative efficiencies for each substrate titration type (relative to the highest efficiency), taken from a minimum of four independent experiments, and error bars represent SDs. Dots indicate significant differences in comparison to results from the respective no-substrate control by Student’s t test (•P < 0.05; ••P < 0.01). (A) Cross-linking in the presence of CL. Experiments were conducted using mitochondria isolated from WT yeast strains. (B) Cross-linking in the absence of CL. Experiments were conducted using mitochondria isolated from the Δcrd1 yeast strain.